Caprisil NH2 HPLC Yopangidwa ndi Chitsulo Chosapanga Dzimbiri Chopangidwa ndi Zitsulo Zosapanga Dzimbiri

Caprisil NH2 Bonded Chromatographic Column imapangidwa ndi silica gel yozungulira yokhala ndi porous ultra-purple ngati matrix, pogwiritsa ntchito aminopropyl ngati matrix yolumikizira, kupereka ultra-high bonding coverage, zomwe zimapangitsa kuti mndandanda wa ma columns awa ukhale ndi retention yayikulu kwambiri, kusonyeza magwiridwe antchito abwino a chromatographic mu ofooka anion exchange, normal phase ndi reversed phase chromatography. Itha kugwiritsidwa ntchito kulekanitsa shuga, nucleotides, alkali compound ndi mamolekyu ambiri a mankhwala. Chifukwa cha njira zosiyanasiyana zolemerera.

Caprisil NH2 ndi yoyenera kugwiritsa ntchito gawo labwino, Caprisil NH2-H ndi yoyenera kugwiritsa ntchito gawo lobwerera m'mbuyo, Caprisil NH2-E stationary phase bonding density ndi yayikulu, zomwe zimapangitsa kuti ogwira ntchito a chromatographic asankhe bwino. Gulu la aminopropyl bond functional la amino column ndi losavuta kulithira hydrolyze kuposa gulu la bond functional la C18 ndi C8 column. Chisamaliro chiyenera kuperekedwa kuti muwongolere kuchuluka kwa pH, pH ikatsika, hydrolysis yoopsa kwambiri idzachitika. Gawo lalikulu la madzi mu gawo loyenda, chiopsezo cha hydrolysis chimakhala chachikulu (osapitirira 50% ya madzi amalimbikitsidwa).

Mzere: Caprisil NH2 250×4.6mm, 5μm

Gawo loyenda: phosphate buffer (0.6g potassium dihydrogen phosphate + 0.279g disodium hydrogen phosphate mu botolo la 1000mL, losungunuka ndi madzi ndikuchepetsedwa ku scale line): acetonitrile (25:75)

Kuchuluka kwa madzi: 2 mL/mphindi

Chowunikira: UV 210nm

Kutentha kwa mzati 35 ℃

Kuchuluka kwa jakisoni: 10 ul

Chitsanzo: 1.acarbose