Mzere wa UPLC Wokhazikika Kwambiri wa Caprisil C18-P

Caprisil C18-P imachotsedwa ntchito mwapadera kuti ipange mawonekedwe apamwamba kwambiri a ma amine ndi ma acid. Caprisil C18-P imagwira ntchito bwino kwambiri chifukwa cha njira yoyambira ndi silica yozungulira yopangidwa ndi ultra-purple synthetically. Tinthu ta silica toyera kwambiri timapangidwa ndi hydroxylated kwathunthu tisanakumane ndi unyolo waukulu wa C18 alkyl, pogwiritsa ntchito njira zomangira. Tinthu ta bonded timeneti timapangidwa kuti tigwirizane ndi magulu ena otsala a silanol omwe amapanga ma columns omwe amalimbana kwambiri ndi kuwonongeka ndi acidic ndi basic mobile phase compositions, pomwe akuwongolera mawonekedwe apamwamba a ma amine ndi ma acid.

Chigawo cha Chromatographic: Caprisil C18-P 250×4.6mm, 5μm

Gawo loyenda: 20mM dipotassium hydrogen phosphate (pH = 7.6)/methanol (30/70)

Liwiro: 1 ml/mphindi

Chowunikira: UV 254nm

Kuchuluka kwa jakisoni: 10 ul

Zitsanzo: gallic acid, p-hydroxybenzoic acid, benzoic acid, Para-methylbenzoic acid

Ubwino Waukulu:

❖ Ukadaulo wopangira ma end-cap awiri kuti ukhale ndi mawonekedwe abwino komanso kuchepetsa kufooka kwa mchira.

❖ Kukhazikika kwa pH (1.5–10) kuti pakhale kulimba komanso kusinthasintha.

❖ Zimasonyeza kuti ukadaulo uwu wapangidwa kuti ugwire ntchito bwino ndi mankhwala oyambira (alkaline).

❖ Kuberekanso bwino kwambiri ndi tinthu ta silika tozungulira.

❖ Njira yogwiritsidwa ntchito kusintha kapena kuteteza magulu otsiriza omwe amakhudzidwa, nthawi zambiri mu ma polima kapena zinthu zopangidwa ndi silica, powaphimba ndi ma reagents enaake.

❖ yoyenera kwambiri kusanthula nthawi zonse.

Mafotokozedwe Onse:

Gawo Losasinthasintha: Octadecylsilyl (C18) kapena Octylsilyl (C8) silica yolumikizidwa.

Kukula kwa Tinthu: 5 µm (masayizi ena amapezeka mukapempha).

Kukula kwa Mabowo: 100 Å (kwabwino kwa mamolekyu ang'onoang'ono mpaka apakatikati).

Miyeso ya Mizati: (makulidwe ena amapezeka ngati mungafune).

Chidutswa chamkati (ID): 4.6 mm (muyezo); komanso 2.1 mm, 3.0 mm, 10 mm zilipo.

Kutalika: 150 mm, 250 mm (muyezo); 50 mm, 100 mm, 200 mm zilipo.

Kuphimba kumapeto: Kuphimba kumapeto kawiri.

Kuchuluka kwa pH: 1.5 - 10.

Pa Ma Column Okonzekera ndi Okonzedwa, tidzapereka kutengera zomwe makasitomala akufuna.