Mzere Wokonzekera wa Caprisil C18-AQ

Caprisil C18-AQ ndi chosungira cha C18 chochuluka kwambiri chomwe chapangidwa makamaka kuti chisunge ma analytes a polar. Caprisil C18-AQ ndi mzati wa gawo losinthika womwe umalola kuti zinthu zosungunuka kwambiri zigwirizane mokwanira ndi gawo la hydrocarbon lolumikizidwa, ngakhale m'magawo oyera amadzi oyenda. Pansi pa mikhalidwe iyi yamadzi oyenda kwambiri, njira yathu yatsopano yolumikizirana imalola maunyolo olumikizidwa kukhalabe otambasuka mokwanira mu gawo loyenda. Caprisil C18-AQ imatha kusunga zinthu zosungunuka kwambiri m'madzi monga ma organic acid ang'onoang'ono, mavitamini osungunuka m'madzi, ma purines ndi ma pyrimidines, ma catecholamines ndi zinthu zina za polar.

Caprisil C18-AQ ndi gulu la madzi lomwe limalimbana ndi gawo la madzi 100%, loyenera kusanthula gawo loyenda la madzi ambiri, lingapereke njira yowunikira mankhwala akuluakulu a polar. Pali magulu okonda madzi pamwamba pa chromatographic packing, omwe amawonjezera kuyanjana. Mukalekanitsa mankhwala a polar mu dongosolo la magawo amadzi ambiri, palibe kusintha kwa makhalidwe osungira omwe adzachitike, ndipo chiwerengero cha mbale ya theoretical ndi magwiridwe antchito a chromatographic peak separation zidzasintha kwambiri.

Gawo loyenda: Madzi a A acetonitrile B 0.1% phosphoric acid

Magiredi:0 mphindi=5%A mphindi 20 =100 %A

Kuchuluka kwa madzi: 1 ml/mphindi

Chowunikira: UV 280nm

Kuchuluka kwa jakisoni: 10ul

Chitsanzo: 1.protocatechuic acid 2.chlorogenic acid 3.ferulic acid 4.coumarin

Mzati: Caprisil C18-AQ 250×4.6mm, 5μm

Gawo loyenda: 50mM H3PO4

Kuchuluka kwa madzi: 1 ml/mphindi

Chowunikira: UV 210nm

Kuchuluka kwa zitsanzo: 5 ul

Chitsanzo: 1. Lactic 2. citric acid 3. fumaric acid 4. gallic acid